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rabbit monoclonal anti p iκb kinase α β  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti p iκb kinase α β
    Rabbit Monoclonal Anti P Iκb Kinase α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/i%CE%BAb+kinase+%CE%B2/pmc12802975-119-10-16?v=Cell+Signaling+Technology+Inc
    Average 95 stars, based on 87 article reviews
    rabbit monoclonal anti p iκb kinase α β - by Bioz Stars, 2026-08
    95/100 stars

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    Protein levels were assessed using Western blotting following stimulation with IL-6 (10 ng/mL) for durations of 10 minutes, 30 minutes, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, and 24 hours in Ca9-22 cells. Densitometric analyses of the Western blot results for ODAM, p-STAT3, STAT3, p-p65, p65, p-IKKα/β, IKKβ, and IKKα are presented. The intensity of the α-tubulin band was normalized to 1, and graphs were generated to illustrate the relative intensities of the other protein bands. IL-6: interleukin-6, ODAM: odontogenic ameloblast-associated protein, STAT3: signal transducer and activator of transcription 3, p-STAT3: phosphorylated STAT3, p65: nuclear factor-kappa B p65, p-p65: phosphorylated p65, IKKα: IκB kinase α, IKKβ: IκB kinase β, p-IKKα/β: phosphorylated IκB kinase α/β. The data represent the mean ± standard deviation from 3 independent experiments, with * P <0.05 and ** P <0.01 indicating statistical significance.
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    Protein levels were assessed using Western blotting following stimulation with IL-6 (10 ng/mL) for durations of 10 minutes, 30 minutes, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, and 24 hours in Ca9-22 cells. Densitometric analyses of the Western blot results for ODAM, p-STAT3, STAT3, p-p65, p65, p-IKKα/β, IKKβ, and IKKα are presented. The intensity of the α-tubulin band was normalized to 1, and graphs were generated to illustrate the relative intensities of the other protein bands. IL-6: interleukin-6, ODAM: odontogenic ameloblast-associated protein, STAT3: signal transducer and activator of transcription 3, p-STAT3: phosphorylated STAT3, p65: nuclear factor-kappa B p65, p-p65: phosphorylated p65, IKKα: IκB kinase α, IKKβ: IκB kinase β, p-IKKα/β: phosphorylated IκB kinase α/β. The data represent the mean ± standard deviation from 3 independent experiments, with * P <0.05 and ** P <0.01 indicating statistical significance.

    Journal: Journal of Periodontal & Implant Science

    Article Title: Interleukin-6 regulates human ODAM gene expression in gingival epithelial cells

    doi: 10.5051/jpis.2402980149

    Figure Lengend Snippet: Protein levels were assessed using Western blotting following stimulation with IL-6 (10 ng/mL) for durations of 10 minutes, 30 minutes, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, and 24 hours in Ca9-22 cells. Densitometric analyses of the Western blot results for ODAM, p-STAT3, STAT3, p-p65, p65, p-IKKα/β, IKKβ, and IKKα are presented. The intensity of the α-tubulin band was normalized to 1, and graphs were generated to illustrate the relative intensities of the other protein bands. IL-6: interleukin-6, ODAM: odontogenic ameloblast-associated protein, STAT3: signal transducer and activator of transcription 3, p-STAT3: phosphorylated STAT3, p65: nuclear factor-kappa B p65, p-p65: phosphorylated p65, IKKα: IκB kinase α, IKKβ: IκB kinase β, p-IKKα/β: phosphorylated IκB kinase α/β. The data represent the mean ± standard deviation from 3 independent experiments, with * P <0.05 and ** P <0.01 indicating statistical significance.

    Article Snippet: These membranes were incubated with primary antibodies: anti-ODAM (PA5-100656; Invitrogen), anti-signal transducer and activator of transcription (STAT) 3 (10253-2-AP; Proteintech, Rosemont, IL, USA), anti-phospho-STAT3 (Tyr705) (ZRB1006; Sigma-Aldrich, St. Louis, MO, USA), anti-nuclear factor-kappa B (NF-κB) p65 (AF5006; Affinity Biosciences, Cincinnati, OH, USA), anti-phosphorylated (phospho-)NF-κB p65 (Ser536) (AF2006; Affinity Biosciences), anti-IκB kinase α (IKKα) (ab32041; Abcam, Cambridge, UK), anti-IκB kinase β (IKKβ) (#2684; Cell Signaling Technology, Danvers, MA, USA), anti-phospho-IKKα/β (Ser176/180) 16A6 (#2697; Cell Signaling Technology), and anti-α-tubulin (sc-5286; Santa Cruz Biotechnology).

    Techniques: Western Blot, Generated, Standard Deviation